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cell culture mouse t cell lymphoma cells  (ATCC)


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    ATCC cell culture mouse t cell lymphoma cells
    Cell Culture Mouse T Cell Lymphoma Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1930 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+culture+mouse+t+cell+lymphoma+cells/EL4/us11162068-190-2-21
    Average 97 stars, based on 1930 article reviews
    cell culture mouse t cell lymphoma cells - by Bioz Stars, 2026-09
    97/100 stars

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    Cell Culture:

    Article Title: Biomimetic support for three-dimensional cell culturing, method for manufacturing same, and use thereof
    Article Snippet: .. Cell and Cell Culture Mouse T cell lymphoma cells (EL4) were purchased from the American Type Culture Collection (ATCC, Manassas, Va., USA). .. EL4 cell lines were cultured in Dulbecco's Modified Eagle Medium (DMEM, HyClone, Logan, Utah, USA) containing 10% (v/v) fetal bovine serum (FBS; Gibco, Invitrogen), 100 IU/mL penicillin (Gibco, Invitrogen) and 100 μg/mL streptomycin (Gibco, Invitrogen) at 37° C. under atmospheric conditions containing 5% CO2.

    Article Title: Biomimetic support for three-dimensional cell culturing, method for manufacturing same, and use thereof
    Article Snippet: .. Cell and Cell Culture Mouse T cell lymphoma cells (EL4) were purchased from the American Type Culture Collection (ATCC, Manassas, Va., USA). .. EL4 cell lines were cultured in Dulbecco's Modified Eagle Medium (DMEM, HyClone, Logan, Utah, USA) containing 10% (v/v) fetal bovine serum (FBS; Gibco, Invitrogen), 100 IU/mL penicillin (Gibco, Invitrogen) and 100 μg/mL streptomycin (Gibco, Invitrogen) at 37° C. under atmospheric conditions containing 5% CO2.

    Article Title: Biomimetic support for three-dimensional cell culturing, method for manufacturing same, and use thereof
    Article Snippet: .. Cell and Cell Culture Mouse T cell lymphoma cells (EL4) and mouse B cell lymphoma cells (A20) were purchased from the American Type Culture Collection (ATCC, Manassas, Va., USA). .. EL4 cell lines were cultured and maintained in Dulbecco's Modified Eagle Medium (DMEM, HyClone, Logan, Utah, USA) containing 10% (v/v) fetal bovine serum (FBS; Gibco, Invitrogen), 100 IU/ml penicillin (Gibco, Invitrogen) and 100 μg/mL streptomycin (Gibco, Invitrogen) at 37° C. under 5% CO2-containing atmospheric conditions.

    Article Title: Biomimetic support for three-dimensional cell culturing, method for manufacturing same, and use thereof
    Article Snippet: .. Cell and Cell Culture Mouse T cell lymphoma cells (EL4) and mouse B cell lymphoma cells (A20) were purchased from the American Type Culture Collection (ATCC, Manassas, Va., USA). .. EL4 cell lines were cultured and maintained in Dulbecco's Modified Eagle Medium (DMEM, HyClone, Logan, Utah, USA) containing 10% (v/v) fetal bovine serum (FBS; Gibco, Invitrogen), 100 IU/ml penicillin (Gibco, Invitrogen) and 100 μg/mL streptomycin (Gibco, Invitrogen) at 37° C. under 5% CO2-containing atmospheric conditions.



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    FIG. 3. Characterization of transformed <t>WR19L</t> cells. Surface expression of human Fas receptor (A) and mouse Fas receptor (B) in WR19L transformants was detected using specific monoclonal antibod- ies and analyzed by flow cytofluorometry. PTP-BL expression in the transformants was analyzed by Western blotting (C). Equal amounts of protein from total lysates were loaded on a 4% polyacrylamide gel, blotted, and PTP-BL was detected using an affinity-purified polyclonal antiserum (a-BL-PTP). Absence (2) or presence (1) of PTP-BL, mouse, and human Fas receptor in the different transformants is indicated at the bottom.
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    FIG. 3. Characterization of transformed WR19L cells. Surface expression of human Fas receptor (A) and mouse Fas receptor (B) in WR19L transformants was detected using specific monoclonal antibod- ies and analyzed by flow cytofluorometry. PTP-BL expression in the transformants was analyzed by Western blotting (C). Equal amounts of protein from total lysates were loaded on a 4% polyacrylamide gel, blotted, and PTP-BL was detected using an affinity-purified polyclonal antiserum (a-BL-PTP). Absence (2) or presence (1) of PTP-BL, mouse, and human Fas receptor in the different transformants is indicated at the bottom.

    Journal: The Journal of biological chemistry

    Article Title: No evidence for involvement of mouse protein-tyrosine phosphatase-BAS-like Fas-associated phosphatase-1 in Fas-mediated apoptosis.

    doi: 10.1074/jbc.272.48.30215

    Figure Lengend Snippet: FIG. 3. Characterization of transformed WR19L cells. Surface expression of human Fas receptor (A) and mouse Fas receptor (B) in WR19L transformants was detected using specific monoclonal antibod- ies and analyzed by flow cytofluorometry. PTP-BL expression in the transformants was analyzed by Western blotting (C). Equal amounts of protein from total lysates were loaded on a 4% polyacrylamide gel, blotted, and PTP-BL was detected using an affinity-purified polyclonal antiserum (a-BL-PTP). Absence (2) or presence (1) of PTP-BL, mouse, and human Fas receptor in the different transformants is indicated at the bottom.

    Article Snippet: Cell Lines and Cell Culture—Mouse T cell lymphoma WR19L cell line (ATCC TIB52) was grown in RPMI 1640 medium containing 10% fetal calf serum.

    Techniques: Transformation Assay, Expressing, Western Blot, Affinity Purification

    FIG. 4. Time response curve of WR19L transformants upon antibody-induced Fas-mediated apoptosis. Apoptosis mediated by the human Fas receptor (A) or the mouse Fas receptor (B) was induced by adding the monoclonal antibody CH-11 (250 ng/ml) or Jo2 (250 ng/ml), respectively. At fixed time points cells were withdrawn, and dead cells were stained by propidium iodine uptake and analyzed by flow cytofluorometry.

    Journal: The Journal of biological chemistry

    Article Title: No evidence for involvement of mouse protein-tyrosine phosphatase-BAS-like Fas-associated phosphatase-1 in Fas-mediated apoptosis.

    doi: 10.1074/jbc.272.48.30215

    Figure Lengend Snippet: FIG. 4. Time response curve of WR19L transformants upon antibody-induced Fas-mediated apoptosis. Apoptosis mediated by the human Fas receptor (A) or the mouse Fas receptor (B) was induced by adding the monoclonal antibody CH-11 (250 ng/ml) or Jo2 (250 ng/ml), respectively. At fixed time points cells were withdrawn, and dead cells were stained by propidium iodine uptake and analyzed by flow cytofluorometry.

    Article Snippet: Cell Lines and Cell Culture—Mouse T cell lymphoma WR19L cell line (ATCC TIB52) was grown in RPMI 1640 medium containing 10% fetal calf serum.

    Techniques: Staining

    FIG. 5. Dose response of WR19L transformants for antibody- induced Fas-mediated apoptosis. Apoptosis was induced in WR19L transformants expressing human Fas receptor (A) and mouse Fas re- ceptor (B) by adding increasing amounts of monoclonal antibody (final concentration ranging from 1029 to 1023 mg/ml). After 20 h dead cells were stained by propidium iodine uptake and analyzed by flow cytofluorometry.

    Journal: The Journal of biological chemistry

    Article Title: No evidence for involvement of mouse protein-tyrosine phosphatase-BAS-like Fas-associated phosphatase-1 in Fas-mediated apoptosis.

    doi: 10.1074/jbc.272.48.30215

    Figure Lengend Snippet: FIG. 5. Dose response of WR19L transformants for antibody- induced Fas-mediated apoptosis. Apoptosis was induced in WR19L transformants expressing human Fas receptor (A) and mouse Fas re- ceptor (B) by adding increasing amounts of monoclonal antibody (final concentration ranging from 1029 to 1023 mg/ml). After 20 h dead cells were stained by propidium iodine uptake and analyzed by flow cytofluorometry.

    Article Snippet: Cell Lines and Cell Culture—Mouse T cell lymphoma WR19L cell line (ATCC TIB52) was grown in RPMI 1640 medium containing 10% fetal calf serum.

    Techniques: Expressing, Concentration Assay, Staining