cell culture mouse t cell lymphoma cells  (ATCC)

Journal: The Journal of biological chemistry

Article Title: No evidence for involvement of mouse protein-tyrosine phosphatase-BAS-like Fas-associated phosphatase-1 in Fas-mediated apoptosis.

doi: 10.1074/jbc.272.48.30215

Figure Lengend Snippet: FIG. 3. Characterization of transformed WR19L cells. Surface expression of human Fas receptor (A) and mouse Fas receptor (B) in WR19L transformants was detected using specific monoclonal antibod- ies and analyzed by flow cytofluorometry. PTP-BL expression in the transformants was analyzed by Western blotting (C). Equal amounts of protein from total lysates were loaded on a 4% polyacrylamide gel, blotted, and PTP-BL was detected using an affinity-purified polyclonal antiserum (a-BL-PTP). Absence (2) or presence (1) of PTP-BL, mouse, and human Fas receptor in the different transformants is indicated at the bottom.

Article Snippet: Cell Lines and Cell Culture—Mouse T cell lymphoma WR19L cell line (ATCC TIB52) was grown in RPMI 1640 medium containing 10% fetal calf serum.

Techniques: Transformation Assay, Expressing, Western Blot, Affinity Purification

Journal: The Journal of biological chemistry

Article Title: No evidence for involvement of mouse protein-tyrosine phosphatase-BAS-like Fas-associated phosphatase-1 in Fas-mediated apoptosis.

doi: 10.1074/jbc.272.48.30215

Figure Lengend Snippet: FIG. 4. Time response curve of WR19L transformants upon antibody-induced Fas-mediated apoptosis. Apoptosis mediated by the human Fas receptor (A) or the mouse Fas receptor (B) was induced by adding the monoclonal antibody CH-11 (250 ng/ml) or Jo2 (250 ng/ml), respectively. At fixed time points cells were withdrawn, and dead cells were stained by propidium iodine uptake and analyzed by flow cytofluorometry.

Article Snippet: Cell Lines and Cell Culture—Mouse T cell lymphoma WR19L cell line (ATCC TIB52) was grown in RPMI 1640 medium containing 10% fetal calf serum.

Techniques: Staining

Journal: The Journal of biological chemistry

Article Title: No evidence for involvement of mouse protein-tyrosine phosphatase-BAS-like Fas-associated phosphatase-1 in Fas-mediated apoptosis.

doi: 10.1074/jbc.272.48.30215

Figure Lengend Snippet: FIG. 5. Dose response of WR19L transformants for antibody- induced Fas-mediated apoptosis. Apoptosis was induced in WR19L transformants expressing human Fas receptor (A) and mouse Fas re- ceptor (B) by adding increasing amounts of monoclonal antibody (final concentration ranging from 1029 to 1023 mg/ml). After 20 h dead cells were stained by propidium iodine uptake and analyzed by flow cytofluorometry.

Article Snippet: Cell Lines and Cell Culture—Mouse T cell lymphoma WR19L cell line (ATCC TIB52) was grown in RPMI 1640 medium containing 10% fetal calf serum.

Techniques: Expressing, Concentration Assay, Staining